脊髓压迫性损伤大鼠海马和脊髓内BDNF的表达变化

摘要

目的 探讨脑源性神经营养因子(BDNF)在脊髓压迫性损伤后的表达变化及作用.方法 用自行设计的方法制作脊髓压迫模型,免疫荧光检测BDNF在星形胶质细胞、神经元和上下行轴突的表达;WB检测BDNF在大鼠海马及脊髓的表达.结果 随着时间延长,受损部位的BDNF+-GFAP荧光强度逐渐增强;BDNF+-Tuj1荧光强度逐渐减弱;受损部位相邻上下段的BDNF+-NF200比受损部位的明显增强.海马和脊髓受损中心相邻的上、下段的BDNF蛋白表达在损伤后1d达到峰值,然后逐渐下降(P<0.05);而脊髓受损中心的BDNF蛋白表达逐渐下降(P<0.05).结论 脊髓损伤后,BDNF的表达下调,随伤后时间呈一定规律变化,是引起受损部位神经元表达下降的原因之一.%Objective To observe changes of BDNF expression in hippocampus and spinal cord of rats after com-pression-induced spinal cord injury .Methods The CSCI model was established with a institution-made device . BDNF in astrocyte, neuron, antegrade and retrograde axons were detected by immunofluorescence .The expression changes of BDNF in hippocampus , lesion centre , the superior segment and the inferior segment which were adjacent to the lesion centre were also determined by Western blot methods .Results The fluorescence intensity of BDNF+-GFAP in lesion centre was increased , while BDNF+-Tuj1 was decreased with time after CSCI .BDNF+-NF200 in superior and inferior segment of lesion was increased .The expression of BDNF in hippocampus , the supe-rior segment and the inferior segment close to the lesion centre reached its peak on the 1st day and then decreased after CSCI(P<0.05).The expression of BDNF in lesion centre decreased gradually (P<0.05).Conclusions De-creasing level of BDNF is regular and may lead to neuronal reduction with compressing time going .