近年来,抗癌药的环境污染特征及其生态风险引起了广泛关注。为获取典型抗癌药5氟尿嘧啶的基础生态毒性数据,以蛋白核小球藻(Chlorella pyrenoidosa)和羊角月芽藻(Selenastrum capricornutum)为受试生物,考察了5氟尿嘧啶对2种绿藻的生长和叶绿素含量的影响。结果表明,5氟尿嘧啶对蛋白核小球藻和羊角月芽藻的生长具有抑制作用,随着暴露浓度升高,细胞生长抑制率增强。5氟尿嘧啶对2种绿藻的96 h半数抑制浓度(EC50)分别为450.36 mg•L-1和692.30 mg•L-1,属于低毒性物质。暴露96 h后,低浓度5氟尿嘧啶(<32 mg•L-1)对蛋白核小球藻和羊角月芽藻叶绿素含量有一定的促进作用,高浓度5氟尿嘧啶(32~500 mg•L-1)则抑制了2种绿藻的叶绿素含量,且两者具有明显的负相关关系。和叶绿素b相比,叶绿素a对5氟尿嘧啶胁迫更为敏感。%The environmental occurrence and ecotoxicological risk of anticancer drugs (also called antineoplastic drugs) have attracted wide attentions. To obtain the basic ecotoxicology data of 5-fluorouracil, a typical anticancer drug, Chlorellapyrenoidosaand Selenastrumcapricornutumwere employed as target organisms, and effect of 5-flu-orouracil on the growth and chlorophyll content of these two algae were examined. The results showed that the growth of C.pyrenoidosa and S.capricornutum were inhibited by 5-fluorouracil. The percentage of growth inhibi-tion was enhanced with the increase of 5-fluorouracil concentration. The 96 h median effective concentration (EC50 ) of 5-fluorouracil to C.pyrenoidosaand S.capricornutumwas 450.36 mg•L-1and 692.30 mg•L-1, respectively. 5-fluorouracil was low toxic to the two algae. After exposure to 5-fluorouracil for 96 h, higher chlorophyll content of C.pyrenoidosaand S.capricornutumwas observed under low concentration of 5-fluorouracil (<32 mg•L-1), while high concentration of 5-fluorouracil (32-500 mg•L-1 ) inhibited chlorophyll a and b content of C. pyrenoidosa and S.capricornutum with a negative correlation. Chlorophyll a is more sensitive to 5-fluorouracil.
展开▼
