毛细管气相色谱测定染发剂中苯二胺

摘要

A capillary gas chromatographic method for simultaneous determination of p-phenylenediamine, m-phenylenediamine,o-phenylenediamine in hair dyes was established.The samples of hair dyes were accurately weighted,selected with the ethylacetate and extracted by the ultrasonic,the impurity was removed by centrifugal work.The extraction was separated by capillary column and detected by FID for qualitativing with retention time and peak area.The linear relation of these three kinds of phenylenedianmine isoma in the range of 0.01 ～ 10 g/L were positive,and the "r" was about 0.9995 ～0.9996.The spiked recovery rates of the o-phenylenediamine, p-phenylenediamine and m-phenylenediamine were 88.6％ ～97.8％ ,93.3％ ～ 105.0％ and 88.5％ ～99.1％.respectively.RSDs were 1.94％ ～2.05％ (n=7) ,and the detection limits were 2,2.5,2 ng/mL, respectively.This method was simple and rapid, which can effectively extract and separate the phenylenediamine isomer in hair dyes with satisfactory results.%建立了毛细管气相色谱法测定染发剂中邻、间、对苯二胺含量的分析方法.样品用乙酸乙酯、超声波提取,离心除去杂质,然后采用毛细管分离,FID检测,保留时间定性,峰面积定量.苯二胺三种异构体在0.01..10 g/L浓度范围内线性关系良好,相关系数为0.999 5～0.9996;邻苯二胺、对苯二胺、间苯二胺加标回收率分别为88.6%～97.8%,93.3%～105.0%,88.5%～99.1%,相对标准偏差为1.94%～2.05%(n=7),检出限分别为2,2.5,2 ng/mL.该方法简单、快速应用于实际样品测定,结果满意.