环丙沙星-铜配合物紫外分光光度法测金属硫蛋白

摘要

In the BR buffer solution ( pH = 6) , CIP-Cu chelate interacted with MTs to lead to enhance the absorbance intensity of system, which was proportional to the concentration of MTs at 272 nm. Based on this phenomenon, a new method for the determination of MTs was proposed. The linear range for MTs was 0.259 ~ 1. 54 |xmol/L with a determination limit of 7.76 x 10 ~8 mol/L. The average recovery was 91.8%. The method is simple, rapid, sensitive, and had been applied to determine trace MTs in human urine with satisfactory results.%在pH =6的BR缓冲溶液中,环丙沙星-铜配合物与金属硫蛋白作用,导致体系吸光强度增强,在272 nm波长处,吸光强度与金属硫蛋白的浓度呈线性关系,据此建立了测定金属硫蛋白的新方法.方法的线性范围为0.259～1.54 μmol/L,检出限7.76×10-8 mol/L,样品平均加标回收率为91.8％.本方法操作简便、快速,灵敏,可用于实际样本测定,结果满意.