HPLC法测定制首乌无糖颗粒中二苯乙烯苷的含量

摘要

建立HPLC测定制首乌无糖颗粒中二苯乙烯苷的含量的方法.色谱条件为Agilent 5TC-C18(150mm×4.6mm,5μm)色谱柱,乙腈-水(27:73)为流动相,流速1.0mL/min,柱温25℃,检测波长320nm,进样量5μL.结果表明,二苯乙烯苷在0.5~5μg范围内线性关系良好(r=0.9996),平均加样回收率为102.22%,RSD=1.08%.该方法简便、可靠,适用于制首乌无糖颗粒的质量控制.%To develop a method for determination of stibene glucoside in radix polygoni multiflori sugar free granula by HPLC.The HPLC analysis was validated on a Agilent 5TC-C18(150 mm×4.6 mm,5 μm)with a mobile phase of acn-water(27:73),the flow rate was 1.0 mL/min,the column temperature was 25℃,the detecting wavelength was 320 nm.The result showed that the stibene glucoside was in good linearity within 0.5~5 μg(r=0.999 6),the average recovery was 102.22%,and RSD was 1.08%.The method is simple and reliable.It can be used for the quality control of radix polygoni multiflori sugar free granula.