为了研究微山湖河蟹养殖区与非河蟹养殖区水体和底泥中异养细菌胞外产酶（蛋白酶、淀粉酶、脂肪酶、脲酶）的差别．对这2处环境中的微生物进行了初步筛选和分离，得到25株菌，分别在含有蛋白质、脂肪、淀粉、脲的固体培养基上培养观察。并测量记录菌落和菌圈大小。结果表明，河蟹养殖区产蛋白水解酶和脂肪水解酶的细菌明显多于非河蟹养殖区，而产淀粉水解酶和脲酶的细菌数量两者相差不大。%In order to study the differences of extracellular enzymes （protease, amylase, lipase, urease） of heterotrophic bacteria in the water body and sediment in cultured area and non--cultured area of crabs in Weishan Lake,microbe in these two environments were preliminarily screened out and separated. And 25 bacterial strains were obtained, cultured and observed on the solid culture media that contained protein, fat, starch and urea. The colonies were recorded and the size of antibacterial circle was measured. The results showed that the bacteria that produced protease and lipase in cultured area of crabs were more than that in non-cultured area of crabs, but the amount of the bacteria that produced amylase and unease had little difference.
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