不同稀释液对藏猪精液4℃保存效果的影响

摘要

This study was conducted to investigate the preservation effect of different extenders on Tibetan Boar semen at 4℃and to provide scientific basis for reasonable and high-efficient utilization of excellent Tibetan Boar breeder semen. Five different extenders commonly used for preservation of Tibetan Boar semen in room temperature were selected. The sperm motility, sperm deformity rate,and the integrity rate of sperm acrosome and plasma membrane preserved by the five extenders at the 5th day of preservation period at 4℃were statistically evaluated and compared. The results revealed that the NO.2 extenders exhibited the best preservation effect with the sperm motility of 0.54 at the 5th day of preservation period, and the sperm motility, sperm deformity rate and the integrity rate of sperm acrosome and plasma membrane preserved with NO.2 extenders were higher than those of the semen preserved with the other four extenders (P<0.05). This study preliminary selected the appropriate extenders for Tibetan Boar semen used at 4℃and laid the foundation for the production and application of Tibetan Boar semen in the future.%为探讨不同精液稀释液对4℃保存藏猪精液的效果，保证优良藏猪种公猪精液的合理、高效利用，该试验选用5种常用的猪精液常温保存稀释液，并在藏猪精液4℃保存至第5天时检查藏猪精子活力、精子畸形率、精子顶体完整率、精子质膜完整率等。结果表明，在精液保存过程中，2号液对藏猪精液的保存效果最佳，精子活力在保存至第5天时仍然保持在0.54，且藏猪精子活力、精子畸形率、精子顶体完整率、精子质膜完整率均高于其余4组（P＜0.05）。该试验研究初步筛选了4℃保存藏猪精液的稀释液，为今后藏猪精液的生产应用奠定了基础。