Aim To evaluate the effects of Tsn the protein level of Collagen Ⅰ and the mRNA levels of Collagen Ⅰ in rats with renovascular hypertension. Methods 2K2C operation was made to SD rats to establish hypertension and LV fibrosis model. Five groups of rats were established from the 4th week after 2K2C : ( 1) sham-operated group treated for 6 weeks with placebo(0. 5％ carboxymethyl cellulose sodium ,solvent for Tsn and Valstarn) ; (2) a group with 2K2C hypertension treated for 6 weeks with placebo(0. 5％ carboxymethyl cellulose sodium) ; (3) a group with 2K2C hypertension treated for 6 weeks with Tsn (70 mg · kg -1 · d-1) ; (4) a group with 2K2C hypertension treated for 6 weeks with Tsn (35 mg · kg-1 · d-1 ) ; (5) a group with 2K2C hypertension treated for 6 weeks with Valsartan (26.7 mg · kg-1 · d-1) . Placebo, Tsn and Valsartan were given by gavage. We measured the protein of Collagen Ⅰ by Western blot analysis , mRNA levels of Collagen Ⅰ, MMP-2 ,MMP-9 .TIMP-1 and TIMP-2 by RT-PCR. Results In contrast to sham-operated group , the mRNA and protein levels of Collagen Ⅰ in 2K2C untreated group were significantly increased. Tsn 70 mg · kg-1 ,35 mg · kg-1 and Valsartan 26. 7 mg· kg - 1 did not suppress the increase in mRNA levels , however , they significantly decreased the protein levels. Conclusion Tsn decreased the Collagen I content on the level of post transtricption or translation.%目的 研究丹参酮Ⅱ A(Tanshinone Ⅱ A,Tsn)对两肾两夹(two-kidney two-clip,2K2C)肾性高血压大鼠心肌I型胶原基因表达、蛋白含量的影响.方法 制作两肾两夹肾性高血压大鼠模型,Tsn 70 mg·kg-1·d-1(高剂量)和35 mg·kg-1·d-1(低剂量)、缬沙坦(Valsartan)26.7 mg·kg-1·d-1连续灌胃给药6周,Western Blot检测心肌组织I型胶原(collagen type I,Collagen I)蛋白表达;RT-PCR检测心肌组织Collagen I水平.结果 Tsn高剂量组、低剂量组和缬沙坦组Collagen I蛋白含量明显低于两肾两夹模型组,分别下降了59.3%、24.6%和63.2%,Tsn高剂量组、低剂量组、缬沙坦组和两肾两夹模型组之间的Collagen I mRNA水平无明显差别,这提示Tsn和缬沙坦对Collagen I mRNA水平无下调作用,二者并不是通过Collagen I基因转录调控以改变胶原含量,而是与转录后和/或翻译后调控有关.结论 Tsn对两肾两夹高血压大鼠左室心肌Collagen I mRNA水平无下调作用,但抑制心肌Collagen I蛋白表达,提示Tsn并不是通过Collagen I基因转录调控以改变胶原含量,而是与转录后和/或翻译后调控有关.
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