富铬松花粉中铬元素的含量测定及降血糖作用研究

摘要

Aim To determine content of chrominum in the chromium-enriched pine pollen, and to explore the hypoglycemic effect of the chromium-enriched pine pollen and sugar tolerance in rats. Methods HN03-HClO4 Wet Digestion was used to process organic chromium sample , and the content of chromium in the chromium-enriched pine pollen was determined by Atomic Ahsorption Spectrophotometry ( AAS ); Model of diabetic rats was established by caudal vein injection of alloxan. After a 15-day intragastric administration of the chromium-enriched pine pollen on these rats, glucose oxidase-peroxidase method was used to determine the glucose level in the blood, while after having received successive intragastric administration of the chromium-enriched pine pollen for 10 days, changes of hlood sugar levels of normal rats were determined pre and post 30, 60 , 120 mins respectively after intragastric administration of glucose solution at the dose of 2. 0 g· kg-1. Results The standard curve of chrominum. tested hy Atomic absorption spectrophotometry, is A = 0. 009 8C +0. 006 2 ; three batches of chromium-rich Pollen content of chromium measured were 16. 775 6 , 17. 682 9 . and 17. 988 3 μg · g-1 respectively. The chromium-enriched pine pollen( 1. 12 g . kg -1 )significantly reduced the blood sugar levels of diabetic rats induced by allox-an. The chromium-enriched pine pollen( 1. 12 g · kg-1 )significantly reduced the hlood sugar levels of normal rats as well, especially remarkable in hypoglycemic effect at 120 min with the chromium-enriched pine pollen( 0. 56 g · kg-1 ). Conclusion The average chromium content of three batches of chromium-rich pine pollen is 17. 482 2 μg · g-1 , and the chromium-enriched pine pollen has the hypoglycemic effect.%目的 测定富铬松花粉中铬元素的含量,探讨富铬松花粉对高血糖大鼠的作用和对大鼠糖耐量的影响.方法 HNO3-HClO4湿式消化法处理有机铬样品,采用原子吸收分光光度法(AAS)检测富铬松花粉中铬元素的含量;尾静脉注射四氧嘧啶诱导高血糖大鼠模型,连续灌胃给药15 d后,葡萄糖氧化酶-过氧化物酶法检测血糖含量;正常大鼠连续灌胃给药10 d后,灌胃葡萄糖溶液(2.0 g·kg-1),于给糖前及给糖后30、60、120 min检测血糖含量变化.结果 采用原子吸收分光光度计测定铬的标准曲线为A=0.009 8C+0.006 2,测得三批富铬松花粉中铬元素的含量分别为16.775 6、17.682 9、17.988 3 μg·g-1.富铬松花粉(1.12 g·kg-1)显著降低四氧嘧啶诱导高血糖大鼠血糖水平.富铬松花粉(1.12、0.56 g·kg-1)显著降低正常大鼠血糖水平,富铬松花粉(0.56 g·kg-1)在120 min时降糖作用明显.结论 三批富铬松花粉中铬元素的平均含量为17.482 2 μg·g-1;富铬松花粉具有降血糖的作用.