目的 建立复方芦丁片中维生素C和芦丁含量的测定方法.方法 采用高效液相色谱法,甲醇-0.05 mol·L-1磷酸二氢钾缓冲液[磷酸调节pH=(3.0±0.1)](45:55)为流动相;流速1.0 ml·min-1;检测波长为254 nm和355 nm双波长测定.结果 维生素C在19.392～242.400 mg·L-1范围内呈良好的线性关系,r=1.000 0,平均回收率为99.14%,RSD=0.69%(n=5);芦丁在9.361 3～117.016 5 mg·L-1范围内呈良好的线性关系,r=0.999 6,平均回收率为98.63%,RSD=1.19%(n=5).结论 该方法简便、准确、重复性好,能同时测定复方芦丁片中维生素C和芦丁的含量.%Objective To establish a method for the determination of vitamin C and rutin in compound rutin tablets. Methods The separation was performed on HPLC. The mobile phase was methanol-potassium dihydrogen phosphate solution( 0.05 mol · L-1 )( adjusted pH to 3. 0 ±0. 1 with phosphoric acid )( 45:55 )at a flow rate of 1. 0 ml · Min-1. The detection wavelength was at 254nm and 355nm. Results The linear of vitamin C was in the range of 19. 392 ~ 242. 400 mg · L-1( r = 1. 000 0 ). The average recovery was 99. 14% and RSD was 0. 69% (n=5). The linear of rutin was in the range of 9. 361 3 ~ 117.016 5 mg · L-1( r =0.9996). The average recovery was 98. 63% and RSD was 1. 19% ( n = 5 ). Conclusion The method is convenient, rapid, accurate and reproducible. It can be suitable for the simultaneous determination of vitamin C and rutin in compound rutin tablets.
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