甲氨喋呤对佐剂性关节炎大鼠滑膜髓样分化因子MyD88表达的影响

摘要

Objective To investigate the change of protein of MyD88 in synovium of adjuvant induced arthritis rats treated with Methotrexate ( MTX) , and also to study the possible mechanism of Methotrexate improving the synovium inflammation . Methods 30 SD rats were randomly assigned into control group (n = 10) and adjuvant induced group (n =20). After modeled, the rats in adjuvant induced group were randomly assigned into model group ( n = 10 ) and Methotrexate group ( n = 10 ). After 4 weeks, HE staining was used to observe synovium pathological changes , IHC and Western blot were used to observe MyD 88 expression, and ELISA was used to measure the ex -pression of inflammation factor IL-1β. Results In the model group , compared to control group , pathological changes of synovium were significant and IL-1β expression improved markedly (P <0.001) , while after treated with MTX,synovium pathological changes and IL -1β expression(P <0. 001)both decreased. Meanwhile ,MyD88 expression was higher in model group than in control group (P <0. 001) ,and treated with MTX,MyD88 expression decreased (P <0. 05). Conclusions The expression of MyD88 in synovium of adjuvant-induced rats increased. MTX decreased its expression and decreased the expression of IL-1β, improved the pathological progress.%目的 探讨甲氨蝶呤对佐剂性关节炎大鼠滑膜组织中髓样分化因子(myeloid differentiation primary response protein,MyD88)的表达的影响及抑制滑膜炎症的可能机制.方法 清洁级SD大鼠30只,随机分为正常组(10只)和完全弗氏佐剂造造模组(20只),造模成功后随机分成2组,模型组和甲氨蝶呤组,每组10只.分别干预4周后,取膝关节滑膜,HE染色观察大鼠滑膜病理改变,免疫组化观察MyD88在滑膜中的定位,Western Blot法检大鼠滑膜中MyD88的表达,EILISA法检测炎性因子IL-1β的表达.结论 模型组大鼠膝关节滑膜组织病理改变明显且炎性因子IL-1β的表达较正常组明显升高(P<0.001),而经甲氨蝶呤治疗后,大鼠滑膜组织病理改变减轻、炎性因子IL-1β的表达降低(P<0.001).模型组大鼠关节滑膜中MyD88表达升高,与正常组相比具有统计学意义(P<0.001),经甲氨蝶呤治疗后MyD88的表达降低,与模型组比较有统计学意义(P<0.05).结论 佐剂性关节炎大鼠滑膜中MyD88的表达明显升高,而甲氨蝶呤可以抑制MyD88的表达从而降低关节滑膜炎症因子IL-1β的表达,改善滑膜增生及炎性因子浸润等病理进程.