Objective To investigate the effects of disodium cantharidinate on gastric cancer cell line SGC-7901 and on the expression of apoptosis-related genes. Methods The gastric cancer cell line SGC-7901 was pretreated with disodium cantharidinate in different dose. The inhibitory rate was detected by MTT assay and expression of Bcl-2 and Bax were detected by RT-PCR and Western blot. Results Disodium cantharidinate showed evident inhibitory action on multiplication of SGC-7901 cells at 2 ～8 mg · L-1 and had a dose-effect relationship. The Bcl-2 decreased and Bax increased were also observed in cells retreated by disodium cantharidinate. Conclusion Disodium cantharidinate can inhibit the growth of SGC-7901 cell lines. The mechanism may be related with induction of cell apoptosis via effect of the expression of apoptosis-related genes such as Bcl-2 by disodium cantharidinate.%目的 观察斑蝥酸钠对人胃癌SGC-7901细胞增殖及细胞凋亡相关基因表达的影响.方法 用斑蝥酸钠处理SGC-7901细胞,分别应用MTT、RT-PCR法和Western blot法检测斑蝥酸钠对细胞的生长抑制率,以及Bcl-2和Bax基因在mRNA和蛋白质水平的表达.结果 斑蝥酸钠在2~8 mg·L-1范围内能抑制SGC-7901细胞增殖,抑制作用呈剂量-时间效应关系,8 mg·L-1斑蝥酸钠处理48 h后的细胞增殖抑制率可达66.3%;斑蝥酸钠能下调凋亡抑制基因Bcl-2的转录和翻译水平,并上调凋亡诱导基因Bax的转录和翻译水平.结论 斑蝥酸钠能抑制胃癌SGC-7901细胞生长,其机制可能与调节控制Bcl-2细胞凋亡相关基因家族表达有关.
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