左西孟旦对犬体外循环术后肺保护的实验研究

摘要

目的：探索左西孟旦（ LS）对实验用杂种犬体外循环（ CPB）术后急性肺损伤（ ALI）是否具有保护作用。方法实验用杂种犬32只，随机分为4组，对照组（C组），氧和冷血肺动脉灌注组（P组），LS静脉注射组（LSIV）；含LS的氧和冷血肺动脉灌注组（ LSP组），LS的剂量均为65 g· kg－1；检测各组肺组织中丙二醛（ MDA）含量、超氧化物歧化酶（ SOD）活性，光镜及电镜观察肺组织的病理形态学变化。结果肺组织中MDA含量及SOD活性，与C组相比，LSP组、P组及LSIV组MDA含量明显降低，且SOD活性明显升高（P＜0．01），与LSP组相比，LSIV组及P组MDA含量明显升高，且SOD活性明显降低（P＜0．01），P组和LSIV组无统计学意义（P＞0．05）。结论通过犬动物实验模型，单纯肺动脉灌注、肺动脉灌注及静脉注射LS，MDA含量明显减少，SOD活性增高，对肺组织具有保护作用。%Objective LS explore experimental surgery with CPB ALI whether dogs have a protective effect.Methods Experiment with 32 dogs were randomly divided into four groups:control group( group C);oxygen and cold-blooded pulmonary perfusion group( P group);LS intravenous group( LSIV);LS containing oxygen and cold-blooded pulmonary perfusion group( LSP group) ,LS doses are 65 g· kg-1;detect the pathological changes of the lung tissue malondialdehyde(MDA),superoxide dismutase(SOD)activity,light mi-croscopy and electron microscopy of lung tissue.Results Lung tissue MDA content and SOD activity,compared with group C,LSP group,P group and MDA LSIV significantly decreased,and SOD activity was significantly increased(P<0.01),compared with the LSP group,LSIV group and P group were significantly increased MDA and SOD activity was significantly lower(P<0.01),P group and LSIV groups was not statistically significant(P>0.05).Conclusion By canine animal models,simple pulmonary perfusion,pulmonary perfusion and intravenousLS,decreased MDA content,SOD activity increased,has a protective effect on lung tissue.