HPLC-ELSD 法测定金线草中β-谷甾醇和胡萝卜苷含量

摘要

Objective To establish an ELSD-HPLC method for the simultaneous determination of β-sitosterol and daucosterol in the herb of Antenoron filiforme.Methods HPLC-ELSD method was adopted with C18 column(250 mm ×4.6 mm,5 μm)and of methanol-water (96∶4)as mobile phase.The flow rate was 1 mL·min -1 ,column temperature was 30℃,the temperature in drift tube was 70℃, the carrier gas flow was 1.8 L·min -1 and the introduction volume was 10 μL.Results The linear ranges of β-sitosterol and daucoste-rol were 0.152 8 ~1.910 μg(r =0.999 5)and 0.101 6 ~1.270 μg(r =0.999 3).The average recovery was 99.7% and 98.9%.RSD (n =5)were 1.4% and 1.8%.Conclusions This method is accurate and reliable for the determination of the above two constituents in the herb of Antenoron filiforme.%目的：建立同时测定金线草中β-谷甾醇和胡萝卜苷的 HPLC 方法。方法采用 HPLC-ELSD 法，色谱柱以十八烷基硅烷键合硅胶为填充剂（250 mm ×4．6 mm，5μm），甲醇—水（96∶4）为流动相洗脱，体积流量1．0 mL·min －1，柱温30℃，漂移管温度70℃，载气体积流量1．8 L·min －1，进样体积10μL。结果β-谷甾醇和胡萝卜苷分别在0．1528～1．910μg（r ＝0．9995）和0．1016～1．270μg（r ＝0．9993）有良好的线性关系；平均回收率为99．7％和98．9％，RSD 分别为1．4％和1．8％（n ＝5）。结论该方法能准确测定金线草中β-谷甾醇和胡萝卜苷的含量。