武当地区马尾松松针中原花青素含量测定研究

摘要

Objective To discuss methods for the content determination of proanthocyandins in pine needles in Wudang area.Meth-ods HPLC -DAD was used to determine proanthocyandin content of pine needles.The Column was Sum Fortis C18 (4.6 mm ×150 mm, 5 μm) with the mobile phase of acetonitrile -water (15∶85).The flow rate was 0.6 mL・ min-1 .The temperature was 30℃, and the detective wavelength was 280 nm.Results The proanthocyandin of pine needles was well separated with other substances. The proanthocyandin’s peak area had a good linear relationship within the concentration range from 0.258 to 2.58 g・ L-1 .The results of methodological study demonstrated that the method met the requirements of the determination.The proanthocyandins contents of pine needles were 44.65 mg・ g-1 .Conclusions The method was simple and effective contents of procyanidins were high, thus providing basis for its further study with promising prospects.%目的：探讨武当地区马尾松松针中原花青素的含量测定方法。方法采用高效液相色谱法测定松针中原花青素的含量。色谱柱为 Sum Fortis C18（4．6 mm ×150 mm，5μm），流动相为乙腈∶水（15∶85），流速为0．6 mL・ min－1，柱温30℃，检测波长为280 nm。结果经方法学考察，原花青素在0．258～2．58 g・ L－1范围内，峰面积与所试浓度线性关系良好，准确度高，精密度好。武当地区松针样品中原花青素含量为44．65 mg・ g －1。结论该方法操作简便，有效成分原花青素的含量较高，开发前景广阔，旨在其进一步研究提供依据。