宫颈上皮内瘤变与人乳头瘤病毒16／18型及沙眼衣原体感染的相关性研究

摘要

目的：探讨人乳头瘤病毒（HPV）16/18型、沙眼衣原体（Ct）与宫颈上皮内瘤变（CIN）的关系。方法采用原位杂交法、免疫层析方法分别对160例宫颈上皮内瘤变（CIN）患者及83例正常宫颈上皮女性行HPV 16/18、Ct检测。结果 CIN组中HPV16/18阳性率为75．00％，与对照组比较，差异有统计学意义（P＜0．05）。在CIN I-～III中，HPV16/18阳性率分别为56．66％、84．31％和87．75％，随着CIN级别的增高，其阳性率呈递增趋势，CIN各组与对照组比较差异有统计学意义（P＜0．05），且CIN II和III较CIN I表达率高，差异有统计学意义（P＜0．05），但CIN II与III两组比较差异无统计学意义（P＞0．05）。CIN组中Ct阳性率为28．75％，与对照组比较，差异有统计学意义（P＜0．05），在CIN I～III中，Ct阳性率分别为23．33％、33．33％和30．61％，CIN各组与对照组比较，差异有统计学意义（P＜0．05），但CIN各级阳性率差异无统计学意义（P＞0．05）。CIN组中HPV16/18合并Ct感染率为13．12％，与对照组比较，差异有统计学意义（P＜0．05）。结论 HPV 16/18感染与CIN病变进展密切相关，Ct感染与CIN的关系值得进一步研究。%Objective Explore the human papillomavirus (HPV)16/18,Chlamydia trachomatis (Ct)and cervical intraepithelial neoplasia (CIN)relationship.Methods Methods of In situ hybridization and Immunochromatography was used to detect the HPV16/18 and Ct in 160 cases of patients with cervical intraepithelial neoplasia and 83 cases of normal cervical epithelium women.Results The positive rate of CIN group HPV16/18 is 75.00%.The result were significantly different compared with the control group (P<0.05).In the CINI-III, HPV16/18 infection rate was 56.66%,84.31%,87.75%,with CIN grade increased,the positive rate showed an increasing trend,CIN in each group compared with the control group were significantly different(P<0.05),and the expressions rate of CINII and III are higher than CINI (P <0 .0 5 ),but the difference between CINII and III has no statistically significant (P >0 .0 5 ).The positive rate Ct of CIN group is 28.75%,significantly different from the control group (P<0.05).In the CINI-III,Ct infection rate was 23.33%、33.33%、30.61%,CIN in each group compared with the control group were significantly different(P<0.05 ),but levels of CIN have no significant difference between the positive rate(P>0.05).The positive rate of consolidation HPV16/18 and Ct was 13.12%in CIN group.The result were significantly dif-ferent compared with the control group(P<0.05).Conclusion HPV16/18 closely associated with CIN lesions progress,Ct infection and CIN relationship deserves further study.