生化糖浆对药物致不完全流产早孕大鼠子宫内膜血管新生及作用机制研究

摘要

Objective To explore the effect of Shenghua syrup on angiogenesis of medicine-induced incomplete-abortion in early pregnancy rats and the related mechanisms. Methods Early pregnancy rats were intragastrically administered with misoprostol and mifepris-tong to establish the incomplete-abortion model. The incomplete-abortion rats were randomly divided into the model group,the positive control group and Shenghua syrup-treated groups. After the successive oral administration for 7 days,blood was collected from aorta abdominalis, and rat uterine tissues were collected. The content of serum Ang-1 and Ang-2 were detected by ELISA;the levels of Tie-2 and MVD in uter-ine tissues were detected by SP immunohistochemistry. Results Endometrial rat model groupˊs MVD and Tie-2 integrated absorbance and se-rum Ang-1,Ang-2 levels were significantly lower than those of the normal control group(P < 0. 05). In positive control group,biochemical syrup dose group,endometrial rat biochemical syrup,MVD in the high dose group and Tie-2 integrated absorbance and serum Ang-1 and Ang-2 levels were significantly higher than those of the model group(P < 0. 05). Conclusion Shenghua syrup has the effect of markedly promoting angiogenesis in incomplete-abortion rats. Its mechanism may be related to the regulation of concentrations of Ang-1 and Ang-2 in serum and Tie-2 in uterine tissues.%目的研究生化糖浆对药物致不完全流产早孕大鼠子宫内膜血管新生及作用机制。方法将受孕7 d 的早孕大鼠灌胃米非司酮和米索前列醇，复制不完全流产模型，将不完全流产模型大鼠分为模型组、阳性对照组、生化糖浆低剂量组、生化糖浆中剂量组和生化糖浆高剂量组，将正常受孕大鼠作为正常对照组。采用免疫组化法检测大鼠子宫内膜中微血管密度（MVD）和酪氨酸激酶受体（Tie-2），采用酶联吸附法检测血清中促血管生成素-1（Ang-1）和促血管生成素-2（Ang-2）。结果 MVD 和 Tie-2积分吸光度以及血清中的 Ang-1和 Ang-2水平显著性的低于正常对照组（P ＜0.05），阳性对照组、生化糖浆中剂量组和生化糖浆高剂量组的 MVD 和 Tie-2积分吸光度以及 Ang-1和 Ang-2水平显著性的高于模型组（P ＜0.05）。结论生化糖浆对药物流产大鼠子宫内膜中的血管新生有显著的促进作用，其作用机制可能与高大鼠子宫中的 Ang-1和 Ang-2以及 Tie-2的表达水平有关。