胎儿正常胰腺组织Stathmin1的免疫组化和原位杂交研究

摘要

Objective To explore the expression of stathmin 1 in the normal fetal pancreatic tissues.Methods From 2012 to 2014,10 fetal specimens of normal pancreatic tissues from dead fetus at different weeks were collected,and the expression of stathmin 1 and its mRNA were detected and observed by immunohistochemistry and in situ hybridization.Results The positive expression rate of stathmin 1 in fetal specimens of pancreatic tissues less than 24 weeks was lower than that of more than 24 weeks (54.72%vs 82.41%,P<0.05),so was it with the positive expression rate of Stathmin 1 mRNA in fetal specimens less than 24 weeks (48.33%vs 73.64%,P<0.05 ).Con-clusion The expression of stathmin 1 exists in normal fetal pancreatic tissues,and its expression increases along with developing fetus.It suggests that stathmin 1 may be involved in the development of fetal pancreatic tissues.%目的：探讨Stathmin1在胎儿正常胰腺组织中的表达情况。方法收集2012至2014年不同周龄胎儿解剖标本中正常胰腺组织10例，应用免疫组织化学法和原位杂交检测 Stathmin1及其 mRNA 的表达。结果＜24周胎儿正常胰腺组织中Stathmin1已有表达，阳性表达率显著低于＞24周胎儿胰腺的正常组织（54.72％vs 82.41％，P＜0.05）；且＜24周胎儿正常胰腺组织中Stathmin1 mRNA也已有表达，阳性表达率显著低于＞24周胎儿胰腺的正常组织（48.33％ vs 73.64％，P＜0.05）。结论胎儿正常胰腺组织Stathmin1已有表达，随着胎儿的发育而发生变化，其可能参与了胎儿胰腺组织的生长发育。