Immunohistochemical Detection of Tumour-Associated Aldehyde Dehydrogenase inFormalin-Fixed Rat and Mouse Normal Liver and Hepatomas

摘要

The paper describes a method and the results of the immunohistochemical (IHC)detection of tumor-associated isozyme of aldehyde dehydrogenase (B-ALDH). The method can be used to demonstrate B-ALDH expression in cells of formalin-fixed, low melting-point paraffin-embedded liver tissue of rats and mice. Thus the method is a substantial improvement over standard histochemical detection methods which require either frozen, or mild preservative-fixed tissue sections. Using the IHC method, B-ALDH expression was detected in hepatocarcinomas of Sprague-Dawley rats treated with the carcinogen diethylnitrosamine (DENA) and in mice treated with either DENA, ethylnitrosourea (ENU), or dichloroacetic acid (DCA). B-ALDH expression has not been previously reported in mice treated with the latter two carcinogens and these results substantiate further the use of B-ALDH as a histochemical marker for mouse hepatocarcinogenesis.