灰绿青霉Penicillium glaucum NS3的诱变育种

摘要

After fermetation conditions optimising of the Penicillium glaucum NS3, a several of synergistic mutation have been carry out for increasing its cellulase - producing capacity. A higher cellulase - producing strain named NS3cl had been gotten, which was breeded through inducing mutation treatmentt of ultraviolet light and sodium nitrite. The optimal dosage of mutant by UV and sodium nitrite was 9 minutes with 15W UV light in 30em distance and 0.3% concentration. Under the repeated synergistic mutations, the CMCase and FPAase produced by mutant Penicillium glaucum NS3cl reached 400.07 IU/ mL and 9.47 IU/mL, which increased 29.47% and 28.35% than that of the original activities 309 IU/mL and 7.37 IU/mL.%以发酵条件优化的菌株Penicilliumglaucum NS3为出发菌，采用紫外线、亚硝酸钠复合诱变，在UV（15W、距30cm辐照9min）、0．3％亚硝酸钠协同诱变条件下，经多轮反复诱变，得到茵株Penicillium glaucum NS3e1，其发酵液CMC酶活和滤纸酶活分别达到400．07IU／mL和9．47IU／mL，比出发茵酶活309Iu／mL和7．37IU／mL分别提高29．47％和28．35％。