沙棘多糖提取工艺研究

摘要

对纤维素酶提取沙棘（HippophaefhamnoidesL．）多糖的工艺进行优化。采用单因素试验考察了酶用量、酶作用的pH、酶作用时间和酶作用的温度对沙棘多糖得率的影响，采用正交试验确定了最佳工艺参数，并与水提法、微波法和超声波的提取效果进行对比研究。超声波辅助提取沙棘多糖的最佳工艺条件为：作用时间50min，酶作用的pH值为5．5，酶用量为2％，酶作用温度为55℃，在此最佳工艺条件下，沙棘多糖得率最高为7．36％。%The study aimed to optimize the Enzymatic extraction technique of polysaccharide from Hippophae fltamnoides L. The effects of extraction time, Enzyme dosage, pH and the temperature enzyme digestion on the yield of polysaccharide were explored by single- factor experiment, and the optimum technique parameter was determined by the orthogonal test. And then the Enzyme method extraction effect of the method was compared with that of water extraction, microwave - assisted ex- traction and ultrasonic assisted extraction. The optimal technique conditions for the extraction were: the extraction time 50min, the extraction temperature 55℃ ,PH 5.5 ,and the Enzyme dosage 2% , under these conditions, the highest yield of polysaceharide from Hippophae fhamnoides L. was 7.36%.