固相支撑液液萃取-气相色谱质谱法测定血液中5种镇静安眠类药物

摘要

To establish a gas chromatography-mass spectrometry(GC/MS) method with supported liquid extraction(SLE) for the determination of common sedative hypnotic drugs in blood. Five sedative hypnotic drugs, amitriptyline, estazolamum,midazolam,promethazine and chlorpromazine,were determined by GC/MS after being processed with SLE, and experimental conditions were optimized. After adding five sedative hypnotic drugs in the blank blood,they were separated and determined by SLE-GC/MS.Optimal experimental conditions were:pH 10 and 5.0 mL of ethyl acetate as the extraction solvent. The results showed a good linearity among the concentrations of 0.5~20 μg/mL of the five drugs and the peak areas with correlation coefficients of 0.998 1~0.999 5,the limits of quantification(LOQ) were approximately 0.5 μg/mL,the average recoveries were 70.1%~90.8%,and the coefficient of variation was less than 5.3%.The method is simple, rapid,with a high recovery,and it can be used in the determination of common sedative hypnotic drugs in blood.%建立了固相支撑液液萃取(SLE)-气相色谱质谱法(GC/MS)提取检验血液中阿米替林、舒乐安定、咪达唑仑、异丙嗪和氯丙嗪5种镇静安眠类药物的方法,对提取条件进行优化,浓缩后的样品溶液使用气相色谱-质谱联用仪分析.5种镇静安眠类药物的最佳提取条件:在pH 10下用5.0 mL乙酸乙酯提取,5种安眠镇静类药物在0.5~20 μg/mL范围内线性关系良好,相关系数在0.9981~0.9995之间,最低检出限为0.5 μg/mL,在血液中的添加回收率在70.1%~90.8%之间,变异系数均小于5.3%.方法操作简便、快捷、回收率高,可用于常见镇静安眠类药物的检验.