普通小麦植株再生系统的优化研究

摘要

Studies were carried out to establish an efficient regeneration system of three bread wheat cultivars. Results showed induction medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) had a higher plantlet regeneration frequency than Piclorm, with an average frequency of 54% in all treatments. Optimal condition for different genotypic rice was as following: induction medium (MSS 3AA/2) with 0.5 mg L-1 2,4-D, regeneration medium (R) with 0.01 mg L-1 2,4-D and 3 mg L-1 KT. The average regeneration frequency reached 83.3% under the condition. Correlation analysis showed that root differentiation, in different level, correlated with green spot regeneration, and with the number of regenerated plants per callus. No correlation was found between green spots regenerated and the numbers of plants regenerated per callus.%为得到高效稳定的植株再生体系,以我国普栽的3个小麦品种幼胚为材料,进行了组培条件的比较优化研究.结果表明,对于植株的再生分化效率,有效浓度的2,4-D作为诱导激素,效果显著高于有效浓度的Piclorm, 所有处理平均可获得54%的植株分化频率.诱导培养基中(MSS 3AA/2)中添加0.5 mg L-1的2,4-D,分化培养基中(R)含0.01 mg L-1的2,4-D配加3 mg L-1的KT 为优化的激素条件,植株平均再生频率可达83.3%.器官分化频率的相关性分析表明:除了绿点的分化频率和每团愈伤组织苗的分化数彼此独立外,苗分化频率、绿点分化频率和每团愈伤组织苗分化数之间都存在不同显著水平的相关性.