转基因马铃薯内源农杆菌脱除

摘要

[Objective] This research aimed to solve the problems of growth and differentiation inhibition of transgenic potato plants caused by antibiotics used for bacteriostasis. [Method] Microtubers were induced using transgenic potato plants, which had generated shoots and formed transgenic bacteria-free plants. [Result] Among the three transgenic potato varieties, the optimal induction medium for SⅠ and SⅡ were MS＋ 0.5 mg/L of 6-BA ＋ 0.1 mg/L of GA3＋ 150 mg/L of cef, and the optimal induction medium for NT were MS＋ 0.5 mg/L of ZT ＋ 0.1 mg/L of GA3 ＋ 150 mg/L of cef; the optimal differentiation medium for tubers were MS＋ 0.5 mg/L of ZT ＋ 0.1 mg/L of NAA, and the tubers with diameters ranging from 0.5 to 0.7 cm had generated the most shoots. The transgenic bacteria-free plants were cultivated in propagation medium without antibiotics for 30 d with a contamination rate of 0, and the stems of bacteria-free plants were stout with no branching. [Conclusion] This method is simple and could be easily applied for the removal of bacteria, which had cleared away obstacles for the selection and growth of transgenic individuals.%[目的]解决采用抗生素抑菌导致转基因马铃薯植株生长与分化受到抑制的问题。[方法]利用转基因植株诱导试管微型薯,由试管微型薯抽枝获得转基因脱菌植株。[结果]参试3个马铃薯品种中,SⅠ、SⅡ最佳试管微型薯诱导培养基为MS＋6-BA0.5mg/L＋GA30.1mg/L＋cef150mg/L,NT最佳试管微型薯诱导培养基为MS＋ZT0.5mg/L＋GA30.1mg/L＋cef150mg/L;薯块抽枝最佳培养基为MS＋ZT0.5mg/L＋NAA0.1mg/L;薯块直径0.5-0.7cm抽枝数最高;转基因脱菌植株在无抗生素快繁培养基中经30d培养污染率为0,脱菌植株茎段粗壮,无分枝现象。[结论]建立的方法脱菌简单易行,为转基因个体的筛选和生长排除了障碍。