3种前体化合物对茅苍术组培苗中主要挥发油成分积累的影响

摘要

[Objective] This study was to explore the effect of three precursors on the accumulation of principal volatile oil constituents in tissue culture plantlets of Atractylodes lancea （Thunb.） DC. with the aim to provide references for the improvement of artificial cultivated A. lancea quality. [Method] Three precursors were added into the MS rooting medium for A. lancea tissue culture plantlets and the volatile oil was extracted by ultrasonication after cultured for several days. The content of atractylon, atractylol, β-eucalyptol and atractydin in the volatile oil were determined by using gas chromatography method. [Result] The addition of xylose, isoprene and tetrahydrofuran impacted the growth indicators, yield of volatile oil and relative percentage content of the four constituents of A. lancea tissue culture plantlets. In the 6 g/L xylose optimized medium, the atractylon and β-eucalyptol content reached up to 4.23% and 56.34%, respectively, 1.41% and 1.66% higher than the control; although the addition of isoprene into medium raised the atractylon content, the accumulation of total volatile oil was inhibited that it decreased by 23.67%, 31.06% and 7.10% to the control; for the tetrahydrofuran optimized medium, the content of atractylon, atractylol and atractydin all increased, and the total volatile oil content increased by 49.97% to the control when the concentration of tetrahydrofuran was 0.07 g/L. [Conclusion] The addition of xylose and tetrahydrofuran promoted the accumulation of principal constituents of the volatile oil, whereas the addition of isoprene inhibited the accumulation.%[目的]研究三种前体化合物对茅苍术组培苗中主要挥发油成分积累的影响，以期为人工栽培茅苍术的品质提高提供帮助。[方法]在茅苍术组培苗MS生根培养基中添加不同化合物，对组培苗进行一段时间培养后，超声提取植株所含的挥发油，利用气相色谱法测定挥发油中苍术酮、苍术醇、β-桉叶醇和苍术素的量。[结果]添加木糖、异戊二烯和四氢呋喃对茅苍术组培苗的生长指标、挥发油得率及四种组分相对百分含量均有影响。6g，L木糖组优化培养基中，检出挥发油中苍术酮、β－桉叶醇的相对百分含量分别达到4．23％和56．34％，分别比对照高出1．41和1．66个百分点；四氢呋喃组优化培养基中苍术酮、苍术醇和苍术素的相对百分含量均有提高，四氢呋喃浓度为0．07g几时总挥发油含量较对照相比增加49，97％；添加异戊二烯到培养基中虽能提高苍术酮的相对百分含量，但对总挥发油含量的积累起到一定的抑制作用，较对照分别减少23．67％、31．06％和7．10％。[结论]添加木糖和四氢呋喃对茅苍术组培苗中主要挥发油的积累具有促进作用，而异戊二烯则具有抑制作用。