在构建楸子叶片SSH cDNA文库及EST分析的基础上,通过电子克隆(in silico cloning)和RT-PCR方法分别从楸子和平邑甜茶叶片中各克隆得到了1个富含甘氨酸RNA结合蛋白(GR-RBP)基因,分别命名为MpGR-RBP1( HM042682)和MhGR-RBP1 (HQ380209).它们的cDNA全长分别为781和558 bp,ORF分别编码171和164个氨基酸的多肽.MpGR- RBP1和MhGR-RBP1基因编码蛋白均含有一个RNA识别结构域(RRM)和一个富含甘氨酸区(glycine-rich region).生物信息学分析表明,MpGR-RBP1和MhGR-RBP1蛋白属于植物GR-RBPs蛋白家族.定量PCR分析表明,MpGR- RBP1和MhGR-RBP1基因在根、茎和叶中均有表达；在干旱胁迫下,它们的表达量增加,而楸子MpGR-RBP1表达水平明显高于平邑甜茶MhGR-RBP1.结果表明,MpGR-RBP1和MhGR-RBP1可能参与植物对干旱胁迫的响应反应,并推测抗旱蛋白结构的差异可能对植物的抗旱性有影响.%In order to investigate the influence of inoculation of Glomus mosseae on the wheat growth under low phosphorus level (1 jimol/L) and high phosphorus level (400 ftmol/L), a single-spore inoculation method was used, and during training sunbags was used to cover the whole pot in order to avoid the influence caused by other fungi. Hie wheat was cultvated in sand supplied with Hogland solution containing different P concentration, and wheat seedlings were inoculated with the spore of Glomus mosseae. The results showed that: compared with the uninoculated wheat, the inoculation increasd the height, root dry weight and plant dry weight of wheat seedings. Whether high phosphorus level or low phosphorus level, inoculated with C. Mosseae fungi, the water potetial of leaf was lower than that of uninoculation; the root activity was increased by 196% (1 /nmol/L P) and 89% (400 ftmol/L); and content of chlorophyll was added; and content of soluble protein was increased clearly in root and leaf. Our conclusion was that wheat and C. Mosseae fungi could form good symbiont.
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