水分胁迫对小麦芽和根中脯氨酸含量及电导率的影响

摘要

The relative conductivity、proline and malondialdehyde(MDA) contents in shoots and roots at seeds germination period of Jinmai 47 (dryland species) and Shunmai 1718 (water species) were compared under water stress that was caused by using 19.2％ PEG6000 for 24 h.The results showed that:after 24 h of water stress,in shoots and roots of wheat,the relative conductivity were increased 22.71 and 31.04 percent respectively in Jinmai 47,and were increased-2.29 ％ and 5.3 ％ respectively in Shunmai ; The membrane injury percentage in Jinmai 47 was greater than in Shunmail718; The same trend was observed for MDA content in shoots and roots,it was increased 23.26％ and 72.55％ respectively in Jinmai 47,and 15.9％ and 61.24％ respectively in Shunmai 1718.Although membrane organizations of two wheat cultivars had being injured,Shunmai 1718 had less injury.Proline contents in shoots and roots of two wheat varieties were significantly increased.For example,it was increased 250.17％ and 75.31％ respectively in Jinmai 47 ; 744.72 ％ and 503.42 ％ respectively,in Shunmai1718.Shunmai 1718 had higher percentage of proline content increase than Jinmai 47 did.As a result,Shunmai1718 would have better protection effect on cell membrane system under water stress than Jinmai 47 did.%采用19.2％ PEG6000模拟水分胁迫24h,比较了晋麦47(旱地品种)和舜麦1718(水地品种)在发芽期芽和根相对电导率、脯氨酸(Pro)含量和丙二醛(MDA)含量等生理指标.结果表明,发芽期水分胁迫24h后,晋麦47芽与根的相对电导率比对照分别增加了22.71％、31.04％,舜麦1718只分别比对照增加了-2.29％、5.3％,晋麦47芽和根的细胞膜伤害率明显大于舜麦1718；且晋麦47在芽和根中的丙二醛含量也分别比对照增加23.26％、72.55％,舜麦1718分别比对照增加15.9％、61.24％,芽和根中的丙二醛含量均低于晋麦47,如相对电导率所示结果一致,说明膜组织都受到损伤,而舜麦1718表现的受损伤程度较轻.同时两个小麦品种的芽和根中脯氨酸含量在水分胁迫下均大幅增加,晋麦47分别增加250.17％、75.31％,舜麦1718分别增加744.72％、503.42％,显著高于晋麦47,这对于舜麦1718在水分胁迫条件下保护细胞具有积极作用.