[Objective]This study aimed to optimize the extraction conditions of high-activity phytase from triticale. [Method]Food and forage triticale 209 was used as an experimental material to investigate the optimal extraction conditions,including pH,solid-liquid ratio,extraction duration and active agent concentration. The extracted phytase was purified with salting-out concentration method for SDS-PAGE electrophoresis Total protein content was measured using Bradford method; phytase activity was measured using vanadium ammonium molybdate method in accordance with the national standard GB/T 18634- 2009. [Result]Phytase activity reached the highest under extraction conditions of pH 5. 0,solid-liquid ratio 10,room temperature,shaking speed 200 r/min and shaking duration1 h,without addition of active agents. [Conclusion]This study improved the extraction technology of phytase from wheat plants and was suitable for practical application.
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