In the present study, Genetic diversity and genetic structure of 7 cultured populations of the HaLiotis diversicolor Reeve from Shantou, Shanwei, Huidong, Shenzhen, Zhanjiang, Xuwen (all are in Guangdong Province, China) was analyzed using 7 microsatellite markers. Sixty eight alleles of these seven microsatellite loci were found in these seven populations. The observed number of alleles of these microsatellite loci was 7~13 in each population. The average number of alleles was 9. 71, and the average effective number of alleles was 3. 8~8. 1. The observed population heterozygosity was 0. 422~0. 906 and the observed expected heterozygosity was 0. 748~0. 917. The polymorphism of the seven populations were all high, and the PIC of the pupations were 0. 657~0. 883 (PJC0. 5). Comparing with two populations of wild domesticated breeding, the five breeding groups showed different degrees of overall reduction in genetic diversity. AMOVA analysis demonstrated that 95. 21% of genentic divergence came from population and 4. 79% came from individuals. These results reavealed that the abalones had a higher level of genetic diversity along the coast of Guangdong province and 7 groups showed a certain some genetic differentiation.%实验选取的7个杂色鲍(Haliotis diversicolor)养殖群体分别来自广东汕头、汕尾、惠东、湛江和徐闻,深圳野生驯养,利用7对微卫星引物对上述群体进行了遗传多样性和群体遗传结构的分析.结果显示:7个位点产生的等位基因数从7～13个不等,共检测到68个等位基因,平均每个位点的等位基因数为9.71个.有效等位基因数的范围为3.8～8.1,平均观测杂合度范围为0.422～0.906,平均期望杂合度范围为0.748～0.917.7个群体的多态信息含量变化范围为0.657～0.883,PIC值均在0.500以上,表现为高度多态性.与两个野生驯养的养殖群体相比,其他5个养殖群体均存在不同程度的遗传多样性降低.AMOVA分析显示,4.79％的遗传变异来自于群体之间,95.21％的变异来自于群体内的个体之间.研究表明,广东沿岸的杂色鲍具有较高的遗传多样性水平,7个群体之间呈现一定的遗传分化.
展开▼
