脱壳病和吻肿病东风螺体内致病菌及条件致病菌菌相研究

摘要

Abstract:The pathogenic bacteria and conditional pathogenic bacteria in the ill and healthy Babylonia areolates were studied by biochemical characterization and 16S rDNA sequence analysis.The results showed that the heterotrophic bacteria in aquatic water for Babylonia areolate attacked by Shell-flesh separating disease were 1.34× 106 cfu /mL,the isolated pathogenic and conditional pathogenic bacteria from ill fish body were Vibrio parahaemolyticus (hemolysis strains),Vibrio harveyi,Vibrio fluvialis,Vibrio hepatarius,Shewanella putrefaciens,Shewanella alga and Bacillus.Vibrio parahaemolyticus (hemolysis strains) and Vibrio Harvey were the dominant species.The heterotrophic bacteria in aquatic water for Babylonia areolate attacked by proboscis edema-disease were 1.46 × 107 cfu/mL,the pathogenic and conditional pathogenic bacteria in infected babylonia areolates were Vibrio parahaemolyticus,Vibrio harveyi,Shewanella abalone,Shewanella alga and Bacillus.The dominant species were Shewanella alga,Shewanella abalone and Vibrio harveyi.The number of heterotrophic bacteria in the water for healthy babylonia areolate was 7.6 × 104 cfu/mL only,Vibrio parahaemolyticus (non-hemolytic strains) and Vibrio hepatarius were the dominanct species in vivo of fish.The results indicated that the accurance of Shell-flesh separating disease and proboscis edema-disease in Babylonia areolate were relevant to the bacteria number in the aquatic environment and also to the internal distribution of pathogenic bacteria and conditional pathogenic bacteria in Babylonia areolate.%为探讨东风螺脱壳病和吻肿病与体内外致病菌及条件致病菌的相关性,测定了东风螺养殖水体环境中异养菌菌落总数,并对病螺及健康螺体内致病菌及条件致病菌进行了分离鉴定.经兔血培养基、TCBS培养基和弧菌显色培养基分离培养,用生化试验和细菌16S rDNA序列分析鉴定,结果显示:发生脱壳病的方斑东风螺螺池水体中的异养菌菌落总数为1.34×106 cfu/mL,病螺体内分离的致病菌及条件致病菌有副溶血弧菌、哈氏弧菌、河流弧菌、Vibrio hepatarius、腐败希瓦氏菌、海藻希瓦氏菌和芽孢杆菌,其中优势菌株为副溶血性弧菌溶血菌株和哈氏弧菌;脱壳病与吻肿病共患的泥螺螺池水体中异养菌的菌落总数为1.46×107 cfu/mL,病螺体内分离的致病菌及条件致病菌有副溶血弧菌、哈氏弧菌、鲍鱼希瓦氏菌、海藻希瓦氏菌和芽胞杆菌,优势菌株为海藻希瓦氏菌、鲍鱼希瓦氏菌和哈氏弧菌;健康东风螺螺池水体异养菌菌落总数为7.6×104 cfu/mL,健康东风螺螺体内的优势菌株为副溶血性弧菌非溶血菌株和Vibrio hepatarius.试验结果表明,东风螺脱壳病和吻肿病与养殖水体环境中异养菌的总数及螺体内部致病菌及条件致病菌分布具有一定相关性.