快速检测痕量铜的光电型传感器的研制

摘要

A photoelectric sensor was composed by home-made portable monochromatic light reflector which inserted a teat strip with immobilized glucose and o-tolidine for copper detection. Principle of the photoelectric sensor was that inhibitory effect of copper on glucose oxidase formed blue substrate by using a coupling reaction system of glucose oxidase- horseradish peroxidase-glucose-o-tolidine. There was good correlative linearity between 1 mg/L ~ 100 mg/L copper solution and light reflecting intensity. The detection limit of method was 0. 064 mg/L, testing time 5 minutes after optimization of the experimental condition.%利用铜对葡萄糖氧化酶的抑制作用,以及葡萄糖氧化酶-辣根过氧化物酶-葡萄糖(Glu) -邻联甲苯胺(OT)偶联反应体系生成蓝色底物的原理,将Glu与OT固定化制成试纸条,插入自制的便携式单色光反射计,组装出检测铜的光电型传感器.当铜的质量浓度范围为1 mg/L ～ 100 mg/L时,光反射强度值与铜的质量浓度呈良好的线性关系.在优化的试验条件下,该方法的检测限为0,064 mg/L,检测时间为5 min.