电针不同穴位对急性尿潴留后家兔膀胱内压和膀胱组织三磷酸腺苷含量的影响

摘要

目的:观察电针“委中”“三阴交”“阴陵泉”穴对急性尿潴留后家兔膀胱内压和膀胱组织三磷酸腺苷(adenosine triphosphate,ATP)的影响,探讨针刺治疗膀胱疾病的优势穴位.方法:选用健康成年雄性家兔48只,随机分为正常组、模型组、委中组、三阴交组、阴陵泉组和非经非穴(腓骨小头后下方)组,每组8只.除正常组外,其余5组均以2倍于正常膀胱容量的生理盐水充盈膀胱2h,建立家兔急性尿潴留模型.2h后排空尿液,即刻电针相应穴位,20 min后通过生物机能信号采集系统观察膀胱内压的变化,用酶联免疫分析法检测膀胱组织ATP含量的变化.结果:与电针前比较,电针后各电针组的膀胱内压明显升高(P＜0.05);与非经非穴组比较,电针后其它电针组膀胱内压的升高率明显偏高(P＜0.05).与模型组和非经非穴组比较,电针后委中、三阴交及阴陵泉组的膀胱ATP含量明显升高(P＜0.05),且委中组的膀胱ATP含量与三阴交和阴陵泉组比较差异亦有统计学意义(P＜0.05),其中委中组升高最为明显,三阴交组次之.结论:电针“委中”“三阴交”及“阴陵泉”穴能显著升高急性尿潴留家兔膀胱内压和膀胱组织ATP的含量,说明委中、三阴交和阴陵泉穴都能有效地治疗急性尿潴留;从改善急性尿潴留家兔的膀胱组织ATP含量来看,“委中”“三阴交”穴效果更明显.%Objective To observe the effect of electroacupuncture (EA) of "Weizhong" (BL 40), "Sanyinjiao" (SP 6) and "Yinlingquan" (SP9) on changes of intravesical pressure and contents of adenosine triphosphate (ATP) in the urinary bladder tissue in rabbits with acute urine retention, so as to explore the relatively specificity of acupoints for urinary bladder problems. Methods Forty-eight male adult rabbits were randomly divided into normal control, model, Weizhong (BL40, EA-BL40), San-yinjiao (SP 6, EA-SP 6), Yinlingquan (SP9, EA-SP9) and non-acupoint (EA-NA) groups- Acute urinary retention model was established by filling the rabbits' bladder with normal saline at a volume of 2 times above their normal capacity for 2 hours. EA (2 Hz/15 Hz, 0. 6 mA) was applied to bilateral "Weizhong" (BL 40), "Sanyinjiao" (SP6) and "Yinlingquan" (SP9) for 20 min, respectively. Intravesical pressure was detected by using a pressure transducer and an amplifier. Bladder ATP content was detected by using enzyme linked immunosorbent assay (ELiSA). Results In comparison with pre-modeling, the intravesicai pressure levels were decreased significantly after modeling in the model, EA-BL 40, EA-SP 6, EA-SP 9 and EA-NA groups (P<0. 05). Compared with pre-EA, intravesical pressure values were increased remarkably in the EA-BL 40, EA-SP 6, EA-SP 9 and EA-NA groups (P<0. 05). The percentages of the increased vesical pressure after EA were considerably higher in the EA-BL 40, EA-SP 6, EA-SP 9 and EA-NA groups than in the model group(P<0. 05). Compared with the normal group, bladder ATP content in the model group was reduced significantly (P<0. 05); while in comparison with the model group, ATP contents in the EA-BL40,EA-SP 6 and EA-SP 9 groups were up-regulated apparently (P<0. 05). The bladder ATP level of the EA-BL 40 group was significantly higher than those of EA-SP 6 and EA-SP 9 groups (P<0. 05). No significant differences were found between the model and EA-NA groups, and between the EA-SP 6 and EA-SP 9 groups in bladder ATP contents (P>0. 05), and among the EA-BL 40, EA-SP 6 and EA-SP 9 groups in the percentages of the increased intravesical pressure (P>0. 05). Conclusion EA of BL 40, SP 6 and SP 9 can significantly raise intravesical pressure and bladder ATP content in urine retention rabbits, which may contribute to its effect in improving urinary retention.