电针“涌泉”“中冲”穴对血管性痴呆大鼠事件相关电位P 300的影响

摘要

目的:探讨事件相关电位P 300在电针“涌泉”“中冲”穴治疗血管性痴呆中的作用机制.方法:采用四血管阻断法复制血管性痴呆大鼠模型,将大鼠随机分为假手术组、模型组、针刺组和西药组,每组12只.针刺组取双侧“涌泉”“中冲”穴,每次电针15 min,西药组予以尼莫地平灌胃(12 mg/kg),2次/d,两组均治疗28d.采用跳台试验记录各组大鼠学习记忆成绩,并采用Neuroscan Nuamps脑电记录分析系统提取各组大鼠事件相关电位P 300成分.结果:模型组大鼠学习成绩表现为反应时间延长、错误次数多,记忆成绩表现为潜伏时间缩短、错误次数多,P 300潜伏期延长、幅值降低,与假手术组比较差异均有统计学意义(P＜0.01)；针刺组及西药组与模型组比较,大鼠学习成绩反应时间缩短、错误次数减少,记忆成绩潜伏时间延长、错误次数减少,P 300潜伏期缩短、幅值升高(均P＜0.01)；针刺组与西药组比较差异无统计学意义(P＞0.05).结论:针刺“涌泉”“中冲”穴可显著改善血管性痴呆模型大鼠的学习记忆成绩及P 300幅值与潜伏期.%Objective To observe the effect of electroacupuncture (EA) stimulation of "Zhongchong" (PC 9) and "Yongquan" (KI 1) on P 300 of event-related potentials (ERPs) in rats with vascular dementia.Methods A total of 48 Wistar rats were randomly divided into sham group (n =12),model group (n =12),EA group (n =12) and medication group (n =12).Vascular dementia model was established by occlusion of the bilateral vertebral arteries and cervical arteries.EA (1-2 mA,2 Hz/20 Hz) was applied to bilateral "Zhongchong" (PC 9) and "Yongquan" (KI 1) for 15 min,once daily for 28 days.Rats of the medication group were treated by intragastric perfusion of Nimodipine (12 mg/kg),twice daily for 28 days.The rats' learningmemory ability was detected by step-down passive and active avoidance tests,and P 300 of ERPs detected by Neuroscan Nuamps system.Results Compared with the sham group,the reaction time and error times of learning performance and error times of memory performance as well as P 300 latency in the model group were significantly increased (P＜0.01),and the latency of memory performance and the amplitude of P 300 were markedly decreased in the model group (P＜0.01).While in comparison with the model group,the reaction time and error times of learning performance,and the error times of memory performance as well as P 300 latencies were obviously decreased in both EA and medication groups (P＜0.01),and the latencies of memory performance and P 300 amplitudes were evidently increased in the EA and medication groups (P＜0.01).No significant differences were found between the EA and medication groups in these 6 indexes(P＞0.05).Conclusion EA of PC 9 and KI 1 can significantly improve the ability of learning and memory and the latency and amplitude of P 300 in rats with vascular dementia.