Objective To investigate the effect of IRF3 on the secretion of inflammatory cytokines IL-6 and TNF-α in hepatic stellate cells induced by LPS. Methods Transient transfection of plasmid pcDNA3-IRF3 into LX-2 of human hepatic stellate cells by liposome was used to detect the transfection efficiency,and the expression of inflammatory factor IL-6 and TNF-α was detected by Western blot and qRT-PCR. Results The results of qRT-PCR and Western blot showed that the expression of TNF-α and IL-6 in LX-2 cells stimulated with 2 μg /ml LPS for 12 h was significantly lower than that in normal control group,while the expression of TNF-α and IL-6 increased significantly. However,after pcDNA3 was transfected into LX-2 cells for 12 h,the expression levels of inflammatory factors IL-6 and TNF-α in pcDNA3 transfected group were significantly inhibited in LX-2 cells compared with the control group. Conclusion IRF3 can inhibit the expression of inflammatory cytokines IL-6 and TNF-α in LX-2 induced by LPS.%目的 研究干扰素调节因子3(IRF3) 对LPS 诱导的肝星状细胞中LX-2 中炎症因子IL-6 及TNF-α 分泌的影响.方法 利用脂质体将质粒pcDNA3-IRF3 瞬时转染到人肝星状细胞LX-2 中过量表达IRF3,qRT-PCR 和Western blot 法检测转染效率,并检测炎症因子IL-6 及TNF-α 的表达.结果 qRT-PCR 和Western blot 结果显示用2 μg /ml 的LPS 刺激 LX-2 细胞12 h 后,与正常组比较,IRF3 的表达量明显降低,而诱导产生的炎症因子TNF-α 及IL-6 的表达量则明显上升.而pcDNA3 转入LX-2 细胞12 h 后,与pcDNA3 空载体对照组比较,pcDNA3 转染组中炎症因子IL-6 及TNF-α 表达量在LX-2 细胞中被显著抑制.结论 在肝星状细胞中, IRF3 能够抑制LPS 诱导的肝星状细胞LX-2 中炎症因子IL-6 及TNF-α 的表达.
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