抗凝剂在改良MAIPA法中对ITP患者血小板特异性自身抗体(GPⅡbⅢa和GPⅠbα)检测的影响

摘要

To investigate the influence of differet anticoagulant by modified monoclonal antibody immobilization of platelet antigen assay ( MAIPA ), on immune thrombocytopenia ( ITP ) platelet specific antibodies glycoprotein IIbI-IIa ( GPIIbIIIa ) and glycoprotein Ibα ( GPIbα ). Samples from ethylene diamine tetraacetic acid dipotassium ( ED-TA-K2 ) and heparin anticoagulation patients were obtained. 162 ITP samples were detected by modified MAIPA platelet specific autoantibodies( GPIIb Ⅲ a and GPIba ). Group of patients were detected. Single anticoagulants group and dual anticoagulants group differences are statistically significant in detection of GPIIbIIIa and GPIbα( P <0. 05 ). There is significant effect in detection of different anticoagulant detected ITP platelet specific antibodies on modified MAIPA. Adopting both anticoagulants to perform combined detection can improve ITP platelet specific antibodies.%探究二价阳离子络合剂的抗凝剂在改良单克隆抗体俘获血小板抗原(MAIPA)法检测免疫性血小板减少症(ITP)患者血小板特异性自身抗体中的影响.分别留取EDTA-K2和肝素钠抗凝的162例ITP患者标本,采用改良MAIPA法检测血小板特异性自身膜糖蛋白IIbIIIa(GPIIbIIIa)和膜糖蛋白Ibα(GPIbα).抗GPIIbIIIa抗体:单用EDTA-K2抗凝剂检出阳性77例,单用肝素钠抗凝剂检出阳性78例,双重检测阳性102例;抗GPIbα抗体:单用EDTA-K2抗凝剂检出阳性53例,单用肝素钠抗凝剂检出阳性51例,双重检测阳性72例.单一抗凝剂与双重抗凝剂比较差异有统计学意义(P<0.05).二价阳离子络合剂的抗凝剂在改良MAIPA法检测ITP患者血小板特异性自身抗体中有一定影响,采用两种抗凝剂双重检测可提高自身抗体的检出率.