兴奋下丘脑腹外侧视前区对结节乳头体核c-Fos表达的影响及其受体途径

摘要

目的 研究兴奋下丘脑腹外侧视前区( VLPO)对结节乳头体核( TMN) c-Fos表达的影响及其受体途径. 方法 免疫组织化学方法检测VLPO代谢型谷氨酸受体5 ( mGluR5 )和TMNγ-氨基丁酸A受体β1亚型(GABAARβ1)的表达;采用脑立体定位技术,观察微量注射谷氨酸( L-Glu)兴奋VL-PO后对TMN c-Fos表达变化的影响. 结果 mGluR5于VL-PO存在阳性表达,同时GABAA Rβ1于TMN存在阳性表达;与对照组相比,VLPO内微量注射L-Glu后,大鼠的TMN区域c-Fos表达量明显减少,差异有统计学意义( P <0. 01 ).结论 存在于VLPO和TMN的mGluR及GABAA R介导VL-PO-TMN的通路调节,VLPO可通过GABAA R抑制TMN神经元活动.%Objective To investigate the effect of c-Fos expression in tuberomammillary nucleus ( TMN) after ex-citing ventrolateral preoptic ( VLPO) and its receptor passway. Methods The expressions of mGluR5 in VLPO and GABAA Rβ1 in TMN were determined by immunostaining. L-Glutamate ( L-Glu) was microinjected into VLPO with stereotaxic technique to observe the changes of c-Fos expression in TMN. Results Positive protein of mGluR5 could be identified by Anti-mGluR5 in VLPO and also positive protein of GABAAR was identified in TMN by Anti-GABAARβ1. c-Fos expression in TMN reduced after microinjecting L-Glu in VLPO compared with the control group. The difference between them was significant (P<0. 01). Conclusion The metabotropic glutamate recep-tor in VLPO and GABA receptor in TMN mediate the regulation of VLPO-TMN passway. VLPO inhibits the TMN neuronal activity via GABAAR.