A procedure for Testing the influence of nucleic acid sequences of the Noncoding region of a Target Gene in a eukaryotic Cell present endogenously on the expression of the GIn diana.

摘要

A procedure for Testing the influence of nucleic acid sequences of the non coding region of a Target Gene in eukaryotic unacu00e9lula endogenously present on the expression of said GENo, Diana.Etapassi procedure that includes the following: (a) the cell is transfected with a Vector for a sequence (i) Control of heterologous expression of active or activated in the cell.Operatively linked to a reporter gene, and (ii) Nucleic Acid Fragments of the region.we Target Gene Non Coding Side 5 and / or Side 3(b) the cell is grown under conditions in which expression control sequence is activated, and (c) measured the expression of the reporter gene.