老龄帕金森病模型小鼠氧化应激相关基因的表达差异

摘要

Objective To explore the behavior changes and oxidative stress related gene expression difference in nigro-striatal system of aging Parkinson’ s disease model mice. Methods Senescence-accelerated mouse prone 8 ( SAMP8) aged 10-month old in sum of 16 were randomly divided into control group and model group. Acute injury was made by subcutaneous injections of 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) to mice in model group, while a corresponding volume of saline was given to the control. 72 hours after injection, all the animals were tested by the behavioral test. Dopamine ( DA) contents in nigro were measured by a high performance liquid chromatography with electochemical detector, and the oxidative stress related genes expression was detected by pol-ymerase chain reaction array ( PCR Array) . Results Compared with the control group, the DA levels and behav-ioral performance were both remarkably decreased in model group ( P2). Conclusion Up-or down-regulation of oxidative stress related genes may result in the occurence of oxidative stress in aging PD model mice.%目的：探讨老龄帕金森病( PD)模型小鼠行为学改变及黑质纹状体系统氧化应激相关基因的表达差异。方法10月龄快速老化P8系( SAMP8)小鼠16只,随机均分为对照组和模型组。模型组小鼠背部皮下注射1-甲基-4-苯基-1,2,3,6-四氢吡啶( MPTP)制成急性损伤模型,对照组小鼠给予同量生理盐水。给药后72 h进行行为学测试,高效液相色谱法检测黑质多巴胺( DA)含量,PCR Array检测其纹状体氧化应激相关基因的表达。结果与对照组相比,模型组小鼠DA水平下降,行为学成绩下降,差异有统计学意义( P2)。结论老龄PD模型小鼠氧化应激相关的基因发生上调或下调,导致氧化应激反应的发生。