肾移植受者早期外周血CD4+ CD25+ FoxP3+ T细胞、Th17细胞表达变化

摘要

Objective To investigate the expression of T regulatory cell( CD4 +CD25 +FoxP3 + T cell or Treg cell) , T help cell 17(Th17 cell) and T cell subsets in renal transplantation recipients during 3 to 6 months after transplan-tation. To analyze the characteristic and significance of these changes. Methods 56 subjects were chosen in this study and they were divided into three groups:group of before kidney transplantation,15 cases;group of 3 to 6 month after transplantation,20 cases;and the normal group,21 cases. The expression percentages of Th17 cell, CD4 +CD25 +FoxP3 +T cell and T cell subsets in PBMC were determined with flow cytometry. Results When com-pared with the group of before kidney transplantation,the group of 3 to 6 months after transplantation had lower ex-pression percentages of CD4 +CD25 +FoxP3 +T cell(P<0. 01) and CD8 +T cell(P<0. 05). When compared with the normal group,the group of 3 to 6 months after transplantation had lower expression percentage of CD4 +CD25 +FoxP3 +T cell(P<0. 001). Conclusion In the group of 3 to 6 months after transplantation,the expression per-centages of CD4 +CD25 +FoxP3 + T cell and CD8 + T cell descend.%目的：观察肾移植受者术后3~6个月外周血单个核细胞( PBMC)中调节性 T细胞( CD4+CD25+FoxP3+T细胞或Treg细胞)、辅助性Th细胞17(Th17细胞)及T细胞亚群的表达百分比的变化情况。分析并探讨上述指标的变化特征及意义。方法收集56例研究对象，分为术前组(15例)，术后3~6个月组(20例)，对照组(21例)。运用流式细胞术检测PBMC中CD4+CD25+FoxP3+T细胞和Th17细胞的表达以及CD4+T细胞和CD8+T细胞的表达。结果与术前组比较，术后3~6个月组CD4+CD25+FoxP3+T细胞的表达低( P<0．01)、CD8+T细胞表达较低( P<0．05)；与对照组比较，术后3~6个月组CD4+CD25+FoxP3+T细胞的表达降低( P<0．001)。结论肾移植术后3~6个月受者外周血中CD4+CD25+FoxP3+T细胞、CD8+T细胞均明显降低。