肝窦内皮细胞诱导T细胞免疫耐受

摘要

Objective To explore the role of LSEC on T cell tolerance. Methods T cell apoptosis, cytokine( IL-2 , IL-4 and IL-10 ) expression and secretion of human T cells activated by CD3/CD28 alone and mixed-cultured with human LSEC were detected. Results The ratio of cell apoptosis was higher in mixed-cultured T cells than T cells alone. The gene expressions of IL-4 and IL-10 were higher in mixed-cultured T cells than T cells alone, while gene expression of IL-2 had no significant difference in mixed-cultured T cells than T cells alone. The cytokine se-cretion of IL-2, IL-4 and IL-10 was consistent with gene expression. Conclusion LSEC could induce T cell toler-ance by decreasing T cell number and altering cytokine secretion.%目的：探讨肝窦内皮细胞( LSEC)对T细胞的耐受作用。方法 CD3和CD28抗体活化的人T细胞和人LSEC混合培养,检测48 h时单独培养和混合培养T细胞的凋亡情况和细胞因子白细胞介素2(IL-2)、白细胞介素4(IL-4)和白细胞介素10(IL-10)的表达量及分泌量。结果和LSEC混合培养的T细胞凋亡率显著高于单独培养的T细胞,混合培养的T细胞中IL-2的表达量没有发生明显变化, IL-4的表达量显著升高,IL-2/IL-4的值降低,IL-10的表达量增加。IL-2、IL-4和IL-10细胞因子分泌情况和表达量一致。结论LSEC 可通过降低T细胞数目和改变细胞因子分泌两种途径诱导T细胞产生耐受。