ADAMTS4、5蛋白酶在人脑胶质瘤中表达及与其恶性程度的关系

摘要

Objective To study the expressions of a disintegrin and metalloprotease with thrombospondin motif 4 and 5(ADAMTS4 and ADAMTS5)proteases in human brain gliomas and their relationship with pathological staging.Methods 44 cases of gliomas of different pathologicail staging, and 11 cases of peri-cancerous brain tissue were selected.Expressions of ADAMTS4 and ADAMTS5 proteases were determined by Envision immunohistochemistry technique.Then the percentage of positive expressed cells was calculated, and the mean optical density(MOD) was measured by the Image Pro Plusversion 6.0(IPP) Image analysis software.Results ADAMTS4 or ADAMTS5 protease was highly expressed in the glioma tissues, which was mainly localized in the cytoplasm and extracellular matrix with brown granular, its expression was lowest in peri-cancerous brain tissue, compared two groups, the difference was statistically(P<0.05).The expression level of ADANTS4 or ADAMTS5 protease gradually increased while the WHO grade of glioma increasing, there was statistically significant difference in comparison with the WHO classification low-grade and high-grade(P<0.05).In 44 cases of brain gliomas, a positive correlation was found between the expression of ADAMTS4 and ADAMTS5 proteases expression(x2=5.614,r=0.357,P<0.05).Conclusion ADAMTS4 and ADAMTS5 proteases expression are markelly increased with the asceding of tumor grade and correlated with the progression and invasiveness of gliomas.In addition, human gliomas tissues, ADAMTS4 and ADAMTS5 proteases expression are positive correlated.The overexpression of them may greatly promote invasiveness and migration of gliomas.%目的 研究人脑胶质瘤中含血小板结合蛋白基序的解聚素金属4(ADAMTS4)蛋白酶和ADAMTS5蛋白酶的表达及其与胶质瘤病理分级之间的关系.方法 采用免疫组化Envision二步法检测ADAMTS4及ADAMTS5蛋白酶在44例不同级别脑胶质瘤、11例瘤周脑组织中的表达,计算阳性细胞百分率,并通过Image Pro Plusversion 6.0(IPP)图像分析软件测量计算其平均光密度(MOD)值.结果 ① ADAMTS4和ADAMTS5蛋白酶在瘤周脑组织中不表达或极低表达,而在胶质瘤组织中高表达,为棕黄色,主要表达定位于细胞外基质及细胞质中,两组差异有统计学意义(P<0.05).进一步研究显示,其蛋白表达量随着肿瘤级别的升高而升高,WHO分级中低级别、高级别间差异有统计学意义(P<0.05).② ADAMTS4与ADAMTS5蛋白酶在胶质瘤中表达呈正相关性(x2=5.614,r=0.357,P<0.05).结论 ① ADAMTS4、ADAMTS5蛋白表达量随着胶质瘤级别的升高而升高,提示了ADAMTS4及ADAMTS5蛋白酶在胶质瘤的进展、侵袭中发挥着重要作用.② ADAMTS4与ADAMTS5蛋白表达呈正相关性,揭示了在胶质瘤的发生发展中这两种蛋白酶可能互相协同,两者的过表达共同促进了胶质瘤的侵袭.