局灶性脑缺血再灌注损伤对小鼠脑组织平滑肌细胞α-SMA表达的影响

摘要

目的 探讨局灶性脑缺血再灌注损伤对小鼠脑组织平滑肌细胞平滑肌肌动蛋白(α-SMA)表达的影响.方法 采用C57BL/6小鼠模型,通过右侧大脑栓塞缺血方法建立局灶性脑缺血再灌注小鼠模型,使用qRT-PCR、Western blot及免疫组织化学等方法,对比观察脑缺血再灌注组小鼠组脑缺血2 h再灌注24 h(实验组)和假手术组及空白对照组大脑组织平滑肌细胞a-SMA的表达情况.结果 氯化三苯四氮唑(TTC)染色结果显示实验组小鼠脑梗死面积明显大于假手术组和空白对照组,实验组小鼠脑水肿明显. Western blot及qRT-PCR结果显示局灶性脑缺血再灌注小鼠组脑缺血2 h再灌注24 h后脑a-SMA的表达明显高于假手术组和空白对照组(P＜0.05).结论 局灶性脑缺血再灌注损伤能刺激小鼠脑组织平滑肌细胞a-SMA的表达.小鼠脑缺血2 h再灌注24 h后脑a-SMA的表达明显高于假手术组和空白对照组.%Objective To investigate the effect of focal cerebral ischemia-reperfusion injury on the expression of a-smooth muscle actin (α-SMA) in mouse brain smooth muscle cells. Methods The middle cerebral artery occlusion (MCAO model) was established by right cerebral embolism ischemia in the C57BL/6 mouse model. The expressions of a-SMA in the smooth muscle cells of the cerebral ischemia reperfusion mice cerebral ischemia 2 h reperfusion 24 h group (the experimental group) and the sham operation group and the blank control group were observed by qRT-PCR, Western blot and immunohistochemistry. Results TTC (2, 3, 5-triphenyltetrazolium chloride )staining showed that the cerebral infarct size of the experimental group was significantly higher than that of the sham operation group and the blank control group. The experimental group had obvious cerebral edema. Western blot and qRT-PCR showed that the expression of a-SMA in focal cerebral ischemia-reperfusion group was significantly higher than that in sham operation group and blank control group at 2 h after reperfusion for 24 h (P ＜ 0. 05). Conclusion Focal cerebral ischemia-reperfusion injury can stimulate the brain smooth muscle cells expression of a-SMA. The expression of a-SMA in brain is significantly higher than that in sham operation group and blank control group at 2 h after reperfusion.