Pretreatraent conditions of detergent method were optimized and contents of NDF (Neutral Detergent Fiber), ADF (Acid Detergent Fiber) and ADL (Acid Detergent Lignin) in tobacco and tobacco products were determined. Results showed; 1) using mixture-solvent (containing benzene, ethanol, and ether) can remove most ester soluble interfering substances in tobacco after 4-hour washing; 2) using ot-amylase can clear up interference of starch during NDF determination; 3) limits of detection of NDF, ADF and ADL are 0.26% , 0.17% , 0.17% , respectively, and the recoveries were all between 89%-101.4% ; 4) differences between contents of NDF and ADF in different types of tobacco leaves are more obvious than in tobacco products; and 5) ADL contents in most tobacco and tobacco products were below 3%.%为准确测定烟草及其制品中各种纤维素含量,优化了洗涤剂法的前处理条件,并定量分析了烟叶及烟草制品中中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)和酸性洗涤木质素(ADL).结果表明:1)前处理中用苯、乙醇、乙醚混合溶剂回流4h能除去绝大部分酯溶性的干扰物质;2)使用高温淀粉酶能够消除烟叶中淀粉对中性洗涤纤维检测带来的干扰;3)NDF、ADF、ADL检测限分别为0.26%、0.17%、0.17%,回收率为89%-101.4%;4)不同类型烟叶原料之间NDF和ADF含量的差异明显高于不同类型卷烟之间的差异;5)烟草及烟草制品的ADL含量基本都在3%以下.
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