斜纹夜蛾核多角体病毒诱导甜菜夜蛾细胞凋亡的研究

摘要

Spodoptera litura nucleopolyhedrovirus (SpltMNPV) could not infect Spodoptera exigua (Se). To investigate the reason of abortive infection, it was detected the pathology of Se301 cells infected by SpltMNPV using different methods, such as microscope, electron microscope and DAPI (4', 6-Diami-dine-2'-phenylindole dihydrochloride) staining, et al. The results demonstrated that the SpltMNPV-in-fected Se301 cells had obvious pathologic changes deduced as early apoptosis character, but these infected Se301 cells did not process to late apoptosis namely forming apoptosis bodies. There was no infectious BV and ODV in the Se301 cells infected by SpltMNPV all the time, suggesting that early apoptosis was still one of the limited factors in viral replication cycle.%斜纹夜蛾核多角体病毒（Spodoptera litura multiple nucleopolyhedrovirus,SpltMNPV）不能成功感染亲缘关系相近的甜菜夜蛾Spodoptera exigua,为了探究其中原因,取SpltMNPV感染甜菜夜蛾离体细胞Se301,利用光学显微镜,电子显微镜以及DAPI (4',6-Diamidine-2'-phenylindole dihydrochloride)荧光染色等方法对其进行检测.结果显示Se301细胞被SpltMNPV感染后出现早期凋亡特征,但未进行至细胞凋亡晚期形成凋亡小体；病毒感染受到阻碍,始终没有病毒多角体和有感染性的芽生型子代病毒产生；综合以上结果说明早期细胞凋亡仍然是限制病毒完成复制周期的重要因素.