转红树总DNA紫花苜蓿T1代耐盐株系的生理生化特性分析

摘要

以花粉管通道法转红树总DNA紫花苜蓿阿尔冈金T1代耐盐植株幼苗为材料,经300 mmol/L NaC1处理后,测定叶绿素、脯氨酸、丙二醛含量和抗氧化酶活性等耐盐相关生理生化指标.结果显示,转基因株系叶绿素含量下降0.09～0.29倍,而对照植株下降0.32倍；转基因株系脯氨酸含量升高1.2～4.2倍,对照植株的脯氨酸升高0.9倍；转基因株系丙二醛含量升高0.15～0.29倍,对照植株的丙二醛含量升高0.36倍；转基因株系超氧化物歧化酶活性升高0.20～0.25倍,对照植株的超氧化物歧化酶活性升高0.19倍；转基因株系过氧化物酶和过氧化氢酶活性分别是对照的1.6～5.6倍和1.2～1.3倍.转基因株系表现出耐盐植物的生理生化特性.%With 300 mmol/L NaCl stress,the physiological and biochemical indexes,including chlorophyll,proline and MDA content,and antioxidase activity,of T1 transgenic alfalfa transformed with Rhizophora apic-ulata total DNA by the pollen-tube pathway,were investigated.The transgenic plantlets had a reduced chlorophyll content of 0.09 -0.29 times,but the non-transgenic plant had 0.32 times.The proline content of transgenic plantlets was enhanced by 1.2 to 4.2 times,but for non-transgenic plant it was 0.9 times.The increased proportion of MDA content was 0.15 -0.29 times in transgenic plantlets compared with 0.36 times for non-transgenic plant.However,the transgenic plantlets had increased SOD activity (0.20-0.25 times) compared with the non-transgenic plant (0.19 times).The POD and CAT activity of the transgenic plantlets were 1.6-5.6 times and 1.2 -1.3 times respectively compared with non- transgenic plants.This suggested that the transgenic plantlets had the physiological and biochemical characteristics of salt tolerant plants.