肌动蛋白与顺铂、反铂的相互作用

摘要

利用1H-NMR研究了肌动蛋白(actin)与顺铂、反铂的相互作用.测定了肌动蛋白的聚合临界浓度,并研究了顺铂、反铂对它的影响.实验结果显示顺铂和反铂都能导致微丝(F-actin)解聚.在相同浓度下,反铂的作用更加明显.对于肌动蛋白单体(G-actin),顺铂在低浓度下促进其聚合,在高浓度下抑制其聚合.反铂则只表现出抑制G-actin的聚合.讨论了顺铂、反铂与G-actin可能的结合位点.顺铂可能与Cys-10和Cys-285结合,而反铂不与Cys-10结合.%The interaction between actin and cisplatin or transplatin has been studied by using the 1H- NMR spectra and measuring the critical concentration of actin polymerization.These studies show that both cisplatin and transplatin induce a depolymerization of F- actin,and that the effect of transplatin is more obvious than that of cisplatin at the same concentration.It is found that cisplatin promotes a polymerization of G- actin at low concentration and inhibits it at high concentration,while transplatin inhibits the polymerization at any concentration.The binding sites of cisplatin / transplatin to G- actin are discussed.It is probable that cisplatin binds with Cys- 10 and Cys- 285,while transplatin does not bind with Cys- 10.