槲皮素对血小板聚集和胞浆游离钙的影响

摘要

AIM: To study the effects of Que on the intraplatelet free calcium concentration and the effects of calcium on the inhibition of platelet aggregation by Que. METHODS: Using Quin-2 fluorescence technique. RESULTS:Que inhibited the platelet aggregation and the rise of [Ca2+]i induced by thrombin in platelets. The values of IC50 and 95 % confidence interval were 146.2 (92.4-231.3)and The inhibitory effects of Que on platelet aggregation induced by thrombin were reduced by adding calcium to the medium, and Que had no effect on thrombin-induced internal Ca2+ release from dense tubular system.on aggregation and the rise of [Ca2+]i in platelets was mainly due to an inhibition of Ca2+ influx.%目的:研究槲皮素对凝血酶诱导的血小板聚集和胞浆游离钙浓度的影响及钙对槲皮素的血小板聚集抑制效应的作用.方法:用荧光钙离子指示剂观察槲皮素对血小板胞浆游离钙的影响.结果:槲皮素明显抑制凝血酶诱导的血小板聚集和游离钙的升高.IC50和95%可信区间分别为146.2(92.4-231.3)和78.5(49.5-124.4)μmol·L-1.槲皮素对血小板的抑制作用可被钙翻转.槲皮素对凝血酶诱导的钙释放无影响.结论:抑制钙内流是槲皮素抑制血小板聚集和[Ca2+]i升高的机制.