人参三醇皂苷对培养心肌细胞的钙通道阻滞作用和抗自由基作用

摘要

To identify the calcium channel blockade and anti-free-radical actions of panaxatriol saponins Re,Rf, Rg1, Rg2, Rh1, and oleanolic acid saponin Ro.METHODS: On ventricular myocytes of Wistar rats, single channel activities of B, L, and T type calcium channels were recorded with the cellattached configuration of patch-clamp technic, and free radical contents were measured with electron spin resenance method. RESULTS: Re, Rg1,Rg2, and Rh1 shortened the open times, prolonged the close times, and reduced the open-state probabilities of B, L, and T type calcium channels; Rf shortened the open time, prolonged the close time and reduced the open-state probability of L type calcium channel; Ro did not influence the activity of and Rh1 antagonized the increase of free radical conRh1 had both the calcium channel blockade and antifree-radical actions. Rf had blockade action on L type calcium channel.%鉴定人参皂苷Re,Rf,Rg1,Rg2,Rh1和R.的钙通道阻滞作用和抗自由基作用.方法:在Wistar大鼠心室肌细胞上,用斑片钳技术记录B、L、T三型单钙通道活动;用ESR法测定自由基含量.结果:Re,Rg1,Rg2,Rh1使B、L、T三型钙通道的开放时间缩短、关闭时间延长、开放率减小,Rf使L型钙通道的开放时间缩短、关闭时间延长、开放概率减小,R.不影响钙通道活动(60 μmol·L-1).Re,Rg1,Rg2,Rh1拮抗Xan0.42 mmol·L-1-XOD 5.3 nmol·L-1诱发的自由基含量增多,Rf和R.无此作用(30 μmol·L-1).结论:Re,Rg1,Rg2,Rh1兼有钙通道阻滞作用和抗自由基作用.Rf对L型钙通道有阻滞作用.