重组葡激酶对中国实验小型猪冠脉血栓作用的研究

摘要

目的:观察重组葡激酶(r-SaK)对中国实验小型猪冠状动脉血栓、心肌缺血、心肌梗塞的影响.方法:直接电刺激中国实验小型猪冠状动脉造成动脉内膜损伤,逐渐形成冠脉内血栓.运用冠状动脉病理切片、显微成像、多媒体图象分析、心外膜电图、心肌组织化学染色、血清生化酶学检查、血液流变学等多种试验手段,研究了r-SaK静脉给药对冠脉血栓的溶栓作用.结果:r-SaK对冠脉血栓有显著的溶栓作用,与对照组比较,r-SaK两个剂量组均能明显缩小冠脉血栓横切面积(P＜0.01),减轻心肌缺血程度和范围(P＜0.01),缩小梗塞区(P＜0.01),降低CPK活性和血液粘度(P＜0.05),抑制血小板粘附、血小板聚集及纤维蛋白原的形成(P＜0.05).结论:r-SaK对冠状动脉血栓具有明显的溶栓作用,并可对抗心肌缺血、心肌梗塞等病理反应.%AIM: To study the effects of recombinant staphylokinase (r-Sak) on coronary thrombosis, cardiac ischemia, and myocardial infarction in Chinese experimental miniature swine. METHODS: Endarterium was injuried and coronary thrombi were formed gradually through direct electrical stimulation on the coronary artery of Chinese experimental miniature swine. Effects of r-Sak in vitro were studied through coronary pathological section,microkinematography, multi media graphic analysis,epicardial electrogram mapping, myocardium histochemical stain, serum creatine phosphokinase (CPK),and hemorheology. RESULTS: r-Sak showed a remarkable effect on coronary thrombus. Compared with the control group, the two dosage groups, r-Sak 0.45rmg/kg and 1.35 mg/kg, reduced the transverse section area of coronary thrombus (P ＜ 0.01 ), lessened the degree and range of cardiac ischemia (P ＜ 0.01 ),decreased the myocardial infarction area ( P ＜ 0.01), the activity of CPK and blood viscosity ( P ＜ 0.05), restrained platelet adhesion and aggregation, and reduced fibrinogen concentration (P ＜ 0.05 ). CONCLUSION: r-Sak could dissolve coronary thrombus obviously and lessen the pathologic reaction of myocardium.