前列腺素A1通过NF-κB介导抑制大鼠心脏微血管内皮细胞凋亡

摘要

NF-κB;细胞凋亡;bcl-2基因;原位切口末端标记目的:研究前列腺素A1(PGAl)对心脏微血管内皮细胞凋亡的影响.方法:培养分离大鼠心脏微血管内皮细胞,建立缺氧再给氧模型,通过原位缺口末端标记观察PGAl对内皮细胞凋亡的作用;通过凝胶电泳迁移率测定NF-κB的活性;用Western blot法测定Bcl-2和Bax蛋白表达;用Northern blot法测定bcl-2 mRNA的表达.结果:PGAl能明显减少缺氧再给氧内皮细胞的凋亡,抑制NF-κB的活性,升高Bcl-2蛋白及bcl-2 mRNA的表达,不改变Bax蛋白的表达,导致Bcl-2/Bax增加.结论:PGAl通过NF-κB介导抑制大鼠心脏微血管内皮细胞的凋亡.%AIM: To study the effects of prostaglandin A1 (PGA1)on rat cardiac microvascular endothelial cells. METHODS: Isolated rat cardiac microvascular endothelial cellswere cultured in hypoxia and reoxygen conditions,respectively. Endothelial cell apoptosis was detected byterminal deoxynucleotidyltransferase-mediated dUTP nickend labeling staining. The activity of NF-κB wasdetected by electrophoretic mobility shift assay. Bcl-2and Bax protein expression were examined by Westernblot and bcl-2 mRNA expression was examined byNortherm blot. RESULTS: PGA1 reduced endothelialcell apoptosis markedly, inhibited activity of NF-κB, andincreased expression of Bcl-2 protein and bcl-2 mRNA.However, PGA1 did not alter Bax protein expressionresulting in an increase in the railo of Bcl-2 to Bax.CONCLUSION: PGA1 can inhibit rat cardiacmicrovascular endothelial cell apoptosis by inhibitingactivity of NF-κB.